克隆化,cloning
1)cloning[英][kl??n][美][klon]克隆化
1.Cloning of spliced variant HBeBP4A of hepatitis B virus e antigen binding protein 4;HBeAg结合蛋白4基因剪切体HBeBP4A的克隆化
2.Cloning of human gene 1 transactivated by DNA polymerase of hepatitis B virus by suppression subtractive hybridization technique;乙型肝炎病毒DNA聚合酶逆转录酶反式调节蛋白1基因的克隆化研究
3.Cloning and identification of gene 8 transactivated by hepatitis C virus non-structural protein 5A;丙型肝炎病毒非结构蛋白5A反式激活基因8的克隆化研究
英文短句/例句
1.Clone Culture of Hybridoma Cells and Purification of Its Secreting McAb;杂交瘤细胞克隆化培养及其分泌的单克隆抗体的纯化
2.Purification, Gene Cloning and Evolutionary Analysis of Conotoxins;芋螺毒素的纯化、基因克隆及进化分析
3.Cloning, Prokaryotic Expression, Purification and Preparation of Human Ferritin Light Chain Polyclonal Antibody;人FTL基因的克隆、表达纯化及其多克隆抗体的制备
4.Clone, Expression, Purification of the Human HBV-TP and Establishment Identification of Anti HBV-TP McAb Hybridoma Cell-lines;乙型肝炎病毒多聚酶末端蛋白的克隆、表达、纯化及单克隆抗体制备
5.Clone, Expression and Purification of AIV Gene PA, M2 and Preparation of Polyclonal Antibody;禽流感病毒基因PA、M2的克隆、表达、纯化及多克隆抗体的制备
6.Construction of the cDNA Clone and Chimeric Clones of Classical Swine Fever Virus C-strain Vaccine;猪瘟兔化弱毒疫苗株cDNA分子克隆与嵌合分子克隆的构建
7.The Cloning and Expression of Human SHP-1 Catalytic Domain and the Preparation of Its Polyclonal Antibody;人SHP-1催化结构域的克隆表达及其多克隆抗体的制备
8.Clone, Expression, Purification of HCV ns5b and Preparation Polyclonal Antibody Against NS5B;丙型肝炎病毒ns5b基因克隆、表达、纯化及多克隆抗体制备
9.Cloning and Expression of Activation Macrophage Membrane Protein AMMP-2 Gene and Preparation of Its Polyclonal Antibody活化巨噬细胞膜蛋白AMMP-2基因的克隆、表达及多克隆抗体的制备
10.Cloning, Expression, Purification and Polyclonal Antibody Preparation of Human Apolipoprotein A5人载脂蛋白A5基因的克隆、表达、纯化及多克隆抗体制备
11.Gene Cloning,Expression and Purification of APL of Keratin 17角蛋白17的置换肽的克隆表达及纯化
12.Cloning, Purification and Expression of Human CD147 and Its Studies on Function;人cd147的克隆、表达、纯化以其功能研究
13.Separation and Purification of Marek"s Disease Tumor-Associated Surface Antigen and Its Monoclonal Antibody Development;MATSA的分离纯化及其单克隆抗体的研制
14.Study of Phytase Gene Being Cloning and Maize Being Transformed;植酸酶基因的克隆及转化玉米的研究
15.The Gene Cloning and Genetic Transformation of Sugarcane Mosaic Virus Coat Protein Gene;SCMV-CP基因克隆及遗传转化甘蔗研究
16.Preparation of the Monoclonal Antibody Against Advanced Glycation End Products;晚期糖基化终产物单克隆抗体的制备
17.Cloning and Phylogenetic Analysis of Sid-1 Genes from Aphids;蚜虫中sid-1基因的克隆与进化分析
18.The Study on the ACC Oxidase Genes Clone of Pear Fruit;梨果实ACC氧化酶基因克隆的研究
相关短句/例句
cloning[英][kl??n][美][klon]克隆，克隆化
3)Clonal differentiation克隆分化
4)cloning DNA克隆化 DNA
1.A new altered sites mutagenesis systems for thecloning DNA is described.本文介绍一种新的变换位点型克隆化 DNA 谤变系统。
5)clonal activation克隆活化
6)DNA cloningDNA克隆(化)
延伸阅读
克隆化克隆化cloning将目的基因与运载体结合后，通过转化引入受体细胞(多用大肠杆菌或枯草杆菌或酵母菌）。使用选择性培养基来筛选出已转化细菌，将筛选出的菌液大量繁殖，这种获取单个细胞培养的过程，通称为克隆化。即细胞通过克隆化可获得“单克隆”细胞系。其目的是分出目的基因细胞，防止竞争淘汰和外来干扰。
